Coding

Part:BBa_K4766013:Design

Designed by: Xinyu Wang   Group: iGEM23_DUT-China   (2023-10-05)


DogCatcher-Mi3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 73
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 73
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 73
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 73
    Illegal AgeI site found at 234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 340


Design Notes

We linked DogCatcher to Mi3 so that it can capture proteins connected with DogTag.

Source

References

W. Kang, X. Ma, D. Kakarla, H. Zhang, Y. Fang, B. Chen, K. Zhu, D. Zheng, Z. Wu, B. Li, C. Xue, Angew. Chem. Int. Ed. 2022, 61, e202214001; Angew. Chem. 2022, 134, e202214001.

Keeble AH, Yadav VK, Ferla MP, Bauer CC, Chuntharpursat-Bon E, Huang J, Bon RS, Howarth M. DogCatcher allows loop-friendly protein-protein ligation. Cell Chem Biol. 2022 Feb 17;29(2):339-350.e10.